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.Download Cheat Plant Vs Zombie Matahari Tak Terbatasl.0170228.ref037]\]. We could not perform a similar evaluation with LAMP because the primers targeting the highly variable *msp2* gene failed to amplify DNA from a few samples from our population.

Therefore, the combination of KAPA2G Robust DNA polymerase and the primers sets was found to be the most appropriate for the amplification of *P*. *vivax*.

A previous evaluation showed that the sensitivity and specificity of the LAMP assay using *pvs25* was higher than that of nested-PCR or RDT \[[@pone.0170228.ref022]\]. With this method, no false negative results were observed, even in samples with low parasitaemia (less than 5 parasites/μL).

A recent study has shown that in combination, the non-recombinant plasmids are the best targets for LAMP assays due to the great template abundance in the samples \[[@pone.0170228.ref012]\]. We then tried to amplify *pvs25* and *pvmr9* from DNA extracted from whole blood and dried blood spots using these two plasmids. Both amplifications were positive after 1 hour in boiling water and 35–50 minutes in isothermal incubation. We did not evaluate *pvama1* in these samples due to the low amount of DNA obtained.

The study conducted by Kobinger *et al* \[[@pone.0170228.ref015]\] on *P*. *falciparum* showed similar results with the assay performed in this study. In their experiments, the optimized protocol was based on the amplification of a conserv

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