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This study investigated the potentials of an image-processing toolbox, BioImageLib [BIOL], to process and reconstruct high-resolution electron micrographs of biological specimens acquired with a 2k x 2k camera. Here, a high-resolution digital electron microscopy technique is introduced and a novel data format, termed the EBSD (electron backscatter diffraction), is developed for serial electron microscopy. A new reconstruction method, called the bilinear interpolation (BIL) reconstruction method, was proposed to reconstruct the atomic resolution images. The effects of various image processing parameters on the BIL reconstruction method were investigated using a 2k x 2k camera by systematically varying the characteristics of the real images. The performance of the proposed reconstruction method was compared with that of the bicubic interpolation (BI) and zero-padding reconstructions (ZP). When we focused on the nuclear structure and cytoplasmic region of the cell because of their high resolution, both the BIL and ZP reconstructions demonstrated better visual quality than did the BI reconstruction. In addition, the BIL reconstruction method produced acceptable results for the cell, mitochondria, microtubules, and virus at the resolution used in this study, and other reconstructions, such as those for the membrane, extracellular matrix, and dendritic spines, were also possible. In conclusion, the BIL reconstruction method seems to be an excellent candidate for use in high-resolution atomic-resolution electron microscopy. The results suggest that the BIL reconstruction method is an efficient method for the reconstruction of
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