Nootaniscphysicsclass12pdffree21 UPD

August 27, 2022 By 0 comment

Nootaniscphysicsclass12pdffree21 UPD



 
 
 
 
 
 
 

Nootaniscphysicsclass12pdffree21

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=”fig”}, **f**) and mean absolute error (MAE) values, as indicated in [Supplementary Table S1](#sup1){ref-type=”supplementary-material”}. Error bars represent mean and s.d.

BioNano Irys system
——————-

Mature microparticles (∼0.5 μm) were immobilized on a glass coverslip, and subsequently stained with fixative solution (0.5 [m]{.smallcaps} sucrose, 0.1 [m]{.smallcaps} imidazole, and 0.05% Triton X-100) for 30 min at room temperature. Fixed microparticles were washed with wash buffer (0.5 [m]{.smallcaps} sucrose, 0.1 [m]{.smallcaps} imidazole and 0.005% Triton X-100) three times before being stored at 4 °C.

Mapping of microparticle centres and diffusion rates
—————————————————–

Imaging of microparticles was performed on a BioNano Irys system (Iris II IN cell, Biomedical Platform, BioNano Genomics) for 15 min. Data from the DNA channel (488 nm) were collected every 20 s. Meta-data were saved using the integrated software, including coordinates of each microparticle, the number of imaging events, the diameter, as well as the location of the microparticle boundaries. Based on the above meta-data, the centre of mass and the average diffusion rate was calculated and exported as Excel files by the integrated software. All data were stored in bam and pst files and integrated into a single dataset using the Integrative Genomics Viewer[@dyu038-B43] (IGV) software. For calculating the exact centre of each microparticle, we first imported the raw Irys data in the IGV software, then the coordinates of the centre of each microparticle were exported as bam files using the ‘Export image’ command in the IGV software. The coordinates were imported into the SeqMonk suite (Version 0.36.2) software[@dyu038-B44] to calculate the centre of each microparticle.

Microscopy
———-

Field emission

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