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Roland SmartDraw Pro CC 2019.Monomer and dimer-bound forms of rabbit skeletal muscle and cardiac troponin T. We have obtained evidence for at least two different forms of rabbit skeletal muscle and cardiac troponin T (cTnT). A protein of approximately 20 kDa can be obtained from sarcoplasmic and myofibrillar extracts of rabbit skeletal muscles by several different protocols, which are likely to produce monomeric and/or dimeric forms of the protein. The 20-kDa form has a high affinity for troponin C (TnC) and is probably a monomer bound to TnC in the complex with the regulatory Ca(2+) subunit troponin I (TnI). Native-gel electrophoresis experiments showed that it was the only form of TnT present in the soluble fraction of skeletal muscle extracts. A second form of TnT (approximately 20 kDa) is obtained by alkaline extraction and contained a small amount of a TnT-TnC-TnI ternary complex. Two-dimensional gel electrophoresis, as well as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of alkaline extracts in the presence of dithiothreitol (DTT), showed the presence of a TnT dimer co-migrating with the monomer and TnT in the ternary complex. The 20-kDa form of TnT was confirmed to be a monomer, whereas the dimer was not. Furthermore, its immunoreactivity was destroyed by DTT treatment, indicating that it is a monomer bound to a disulfide-linked complex of TnT. The observed electrophoretic mobility was the same as that of the 20-kDa form in the skeletal muscle extracts. It seems likely that the cTnT dimer is a dimer of TnT bound to two TnI molecules. The larger form (20 kDa) is probably a dimer of two TnT monomers bound to a single TnI molecule.Q: Simple exercise to calculate 2pi times radius of convergence I have a simple exercise about the radius of convergence of an expansion, but I don’t know how to solve it. Could someone help me? In a center $(x_{c},y_{c})

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